Detection of Antimicrobial Resistance Genes by Using Molecular Methods in Urinary Tract Infections (UTI) patients in the Babil Site
Keywords:
Beta-Lactamase, Bla-TEM, Bla-AMPCAbstract
Around the world, there is growing worry over beta-lactamase resistance in Enterobacteriaceae, specifically in Escherich coli (E. coli). The present study aimed to determine the frequency of the beta-lactamase-resistant bla-TEM and bla-AMPC genes in the Enterobacteriaceae family using PCR. Thirty isolates from the Babil Teaching Hospital were obtained for identification since E. coli beta-lactamase genes were discovered in certain laboratories among the 71.6 positive isolates. The current study's findings demonstrated that patients (2 to 18 years old) constituted the majority of the isolates of beta-lactamase-resistant E. coli. Across all bacterial isolates, bla-AMPC and bla-TEM were significantly more common than bla-CTX-M and bla-SHV-5 (
). bla-AMPC and bla-TEM had prevalence rates of 93.33% and 71.66%, respectively, but bla-SHV-5 and bla-AMPC had lower rates of 20% and 46.66%, respectively. In certain pathogenic bacteria, the potential relationships between virulence genes and antibiotic resistance were investigated. Considering Given that the existence of established infectious factors in clinical isolates could potentially facilitate infection and pathogen persistence, virulence factors were analyzed in relation to these genes and a few additional genes that were studied in the same lab. The current study demonstrated a statistically significant association between antibiotic resistance profile and virulence, with a person correlation of 0.957 between virulence and antibiotic determinants, according to the statistical analysis. The biofilm gene, which was found in 95% of the bacterial isolates, was the most prevalent gene according to the gene virulence data, followed by the iss gene (86.6%) and the fimH gene 85%. Therefore, the discovery of a relationship between virulence profile and resistance may contribute to the development of various bacterial infection rates and their recurrence in the clinical setting.
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